SIMULTANEOUS DETERMINATION OF VIABILITY AND ACROSOMAL STATUS,. OF BOVINE SPERMATOZOA BY DUAL STAINING TECHNIQUE

Abstract

With the aim of simultaneously determining the viability and acrosomal status of bovine spermatozoa, the two common staining techniques, Nigrosin-Eosin (NE) staining and the Giemsa staining technique, were combined. The proportions of live bovine spermatozoa as determined by the NE technique and the Nigrosin-Eosin-Giemsa (NEG) technique were similar. Acrosomal status determined by the NEG technique did not differ significantly from that of the Giemsa staining technique. This staining technique for
identifying live and dead bovine spermatozoa and acrosomal status in the same smear is capable of determining four categories of
spermatozoa, live acrosome intact, live acrosome-reacted or damaged, dead acrosome intact and dead acrosome-reacted or damaged.